金克隆生物公司位于北京經(jīng)濟(jì)技術(shù)開發(fā)區(qū)，是一家集研發(fā)、銷售和服務(wù)于一體的高新技術(shù)企業(yè)。擁有五千平米的研發(fā)和倉儲(chǔ)基地，自產(chǎn)試劑及常備生化試劑耗材等近兩萬種現(xiàn)貨庫存。涵蓋細(xì)胞學(xué)、分子生物學(xué)、蛋白免疫學(xué)、組織學(xué)、微生物培養(yǎng)等。
我公司金克隆有專業(yè)的技術(shù)團(tuán)隊(duì)和質(zhì)檢團(tuán)隊(duì)。所有產(chǎn)品，技術(shù)團(tuán)隊(duì)負(fù)責(zé)全程的技術(shù)指導(dǎo)分析。每個(gè)產(chǎn)品，質(zhì)檢團(tuán)隊(duì)反復(fù)檢測，合格后才會(huì)入庫銷售，出售前質(zhì)檢充分，免除客戶質(zhì)量之憂。

金克隆生物產(chǎn)品覆蓋系列如下：
1 細(xì)胞學(xué)：細(xì)胞培養(yǎng)、細(xì)胞分離、細(xì)胞檢測等各種細(xì)胞實(shí)驗(yàn)的試劑耗材盒子。
2 分子生物學(xué)：核酸提取純化、PCR、核酸電泳、克隆重組表達(dá)、測序和文庫構(gòu)建、生化檢測等各種分子實(shí)驗(yàn)的緩沖液試劑耗材盒子。
3 蛋白免疫學(xué)：裂解、蛋白純化、層析填料、蛋白定量、凝膠制備、蛋白電泳、顯色曝光、免疫檢測、抗原抗體ELISA等各種蛋白免疫實(shí)驗(yàn)的緩沖液試劑耗材盒子。
4 組織學(xué)：切片爬片、固定染色（細(xì)胞染色、組織染色、熒光染色）等各種試劑耗材盒子。我公司染色產(chǎn)品均使用進(jìn)口原料，染色清晰，批次無差異。
5 微生物培養(yǎng)：菌株、微生物培養(yǎng)（酵母缺陷型培養(yǎng)基）、發(fā)酵產(chǎn)品、微生物檢測（藥敏紙片）等各種微生物相關(guān)的試劑耗材盒子。
6 生化檢測試劑盒：現(xiàn)有26個(gè)系列,用途涵蓋醫(yī)、藥、農(nóng)、林、畜牧、水產(chǎn)、體育、生物、環(huán)保、營養(yǎng)、食品衛(wèi)生、預(yù)防醫(yī)學(xué)、保健品及化妝品等。
7 試劑系列：生化試劑，緩沖劑，標(biāo)準(zhǔn)品，抗生素，酶，小分子抑制劑，熒光染料，無機(jī)鹽等近萬種產(chǎn)品庫存，產(chǎn)品全，價(jià)格低，質(zhì)量好。

金克隆生物有限公司專業(yè)生化試劑生產(chǎn)廠家，品類齊全，價(jià)格優(yōu)勢，質(zhì)量保證，涵蓋生物學(xué)、化學(xué)、醫(yī)藥學(xué)等各專業(yè)所需試劑耗材，并代理sigma、amersco、羅氏、qiagen、gibco、NEB、MBI等各種進(jìn)口試劑，歡迎垂詢訂購！

金克隆生物公司位于北京經(jīng)濟(jì)技術(shù)開發(fā)區(qū)，主要從事生物醫(yī)藥試劑的研發(fā)和生產(chǎn)，并兼營部分生化試劑和儀器耗材。其中我公司自主開發(fā)的分子生物學(xué)試劑、細(xì)胞培養(yǎng)及檢測試劑、分子免疫學(xué)產(chǎn)品等在質(zhì)量和價(jià)格方面具有較大的市場優(yōu)勢，歡迎廣大經(jīng)銷商洽談合作。

金克隆（北京）生物技術(shù)有限公司，專業(yè)生產(chǎn)細(xì)胞培養(yǎng)試劑，專業(yè)生產(chǎn)無菌試劑，GMP標(biāo)準(zhǔn)DMEM培養(yǎng)基，1640培養(yǎng)基，配套試劑及緩沖液PBS，hanks等，以及實(shí)驗(yàn)室常用DNA、蛋白MARKER，電泳試劑等. 金克隆生物有限公司專業(yè)生化試劑廠家，本公司現(xiàn)貨產(chǎn)品近一萬種，定期會(huì)有優(yōu)惠活動(dòng)，詳情請(qǐng)聯(lián)系客服人員歡迎垂詢!

GcloneGelGreen Nucleic Acid Gel Stain (10000×)

Catalog Number: AA0115

Size: 0.5 mL

Storage:

GcloneGelGreen is stable for at least one year when protected from light, at room temperature. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of precipitate on the surface of the gel. If this occurs, heat the solution to 45-50℃ for two minutes and vortex.

Product Description:

GcloneGelGreen is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GcloneGelGreen has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm. Thus, GcloneGelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader).

GcloneGelGreen is far more sensitive than SYBR® Safe. Unlike SYBR® dyes, which are known to be unstable, GcloneGelGreen is very stable, both hydrolytically and thermally. Unlike the highly mutagenic EtBr and the reportedly mutation-enhancing SYBR® Green I, GcloneGelGreen is noncytotoxic and nonmutagenic at concentrations well above the working concentrations used in gel staining, because of the dye’s inability to cross cell membranes.

Gel staining with GcloneGelGreen is compatible with downstream applications such as gel extraction and cloning. It is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation. But it is not designed for qPCR applications, for which we recommend EvaGreen dye.

Staining Protocols:

Because nucleic acid binding dyes can affect DNA migration during electrophoresis, post-staining of gels is highly recommended. Post-staining with GcloneGelGreen results in superior sensitivity and eliminates the possibility of dye interference with DNA migration. Post-staining with GcloneGelGreen is simple, requiring no destaining and no special buffer. GcloneGelGreen also can be included in agarose gels using the precast method. While the precast protocol is more convenient, some DNA samples may experience migration retardation or compromised resolution in the presence of GcloneGelGreen. Thus, the post-staining and precast protocols should be compared to determine which one better meets your needs.

Although GcloneGelGreen has undergone extensive safety testing, we recommend following universal safety precautions when working in the laboratory.

1. Post-staining Protocol

1.1 Run gels as usual according to your standard protocol.

1.2 Dilute the GcloneGelGreen 10000× stock reagent 3300 fold to make a 3× staining solution in H2O.

Note: including 0.1 M NaCl in the staining solution enhances sensitivity, but may promote dye

precipitation if the gel stain is reused.

1.3 Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3× staining solution to submerge the gel.

1.4 Agitate the gel gently at room temperature for 30 minutes.

1.5 Image the stained gel with a 254 nm transilluminator, a Dark Reader® or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.

1.6 Staining solution can be reused at least 2-3 times. Store staining solution at room temperature protected from light.

2. Pre-cast protocol

2.1 Prepare molten agarose gel solution using your standard protocol.

2.2 Dilute the GcloneGelGreen 10000× stock reagent into the molten agarose gel solution at 1:10000 and mix thoroughly. GcloneGelGreen can be added while the gel solution is still hot.

2.3 Cast the gel and allow it to solidify. Any leftover gel solution may be stored and re-heated later for additional gel casting. GcloneGelGreen precast gels may be stored at 4℃ for later use.

2.4 Load samples and run the gels using your standard protocol.

2.5 Image the stained gel with a 254 nm transilluminator, a Dark Reader® or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.

2.6 Note: The pre-cast protocol is not recommended for polyacrylamide gels. Use the post staining protocol for acrylamide gels.