北京優(yōu)尼康生物科技有限公司主要經(jīng)營(yíng)進(jìn)口、國(guó)產(chǎn)生物科研儀器、試劑及耗材，提供技術(shù)服務(wù)的生物公司之一?，F(xiàn)經(jīng)營(yíng)的品牌及相關(guān)產(chǎn)品如下：

德國(guó)Griner培養(yǎng)板、培養(yǎng)皿、離心管、凍存管、培養(yǎng)瓶、接種環(huán)、接種針等；

美國(guó)Wheaton 勻漿器、組織研磨器、雙側(cè)壁細(xì)胞培養(yǎng)瓶、懸浮細(xì)胞培養(yǎng)瓶、細(xì)胞專用攪拌器、安培瓶等玻璃儀器。

美國(guó)kimble 藍(lán)蓋瓶、勻漿器、離心管，巴氏吸管等玻璃儀器；

加拿大Labplas 的無(wú)菌采樣袋、均值袋；

美國(guó)Nalgene 過(guò)濾裝置、塑料廣口瓶、放水桶、低溫冰盒、低溫標(biāo)簽、記號(hào)筆、離心管、凍存管、便攜液氮罐、無(wú)菌培養(yǎng)基瓶、手動(dòng)真空泵等

美國(guó)ABI PCR板、PCR管、PCR蓋子、八連拍管、膜等

德國(guó)Brand 移液器，電動(dòng)移液器，瓶口分配等

德國(guó)艾本德eppendorf 移液器、分配器等

法國(guó)Hyphen Biomed 的出血、凝血類試劑 ；

美國(guó)BioProducts MD 的PEDF(人眼色素上皮衍生因子)；

德國(guó)IBL international 新蝶呤試劑盒、兒茶酚胺試劑盒；

美國(guó)TYLER 的篩分儀；

BD公司的基質(zhì)膠,細(xì)胞篩網(wǎng)等;

法國(guó)Interscience 均質(zhì)器、重量稀釋器、自動(dòng)螺旋接種儀、均值袋、均值夾、開袋器、架子等

德國(guó)Wiggens 攪拌器、培養(yǎng)箱、振蕩器、蒸發(fā)器（氮吹儀）菌落計(jì)數(shù)器本生燈、均質(zhì)器等

德國(guó)Chemvck 防腐隔膜泵、無(wú)油泵、真空泵、蠕動(dòng)泵等

瑞士Keller 小型快速玻璃珠滅菌器、玻璃珠等

美國(guó)Millpore 細(xì)胞電阻儀、超濾管、膜、針頭濾器等

美國(guó) BioSpec研磨珠均質(zhì)器、手持勻漿機(jī)等

自產(chǎn)鎳洛合金接種環(huán)、接種針、核酸染料等

CHIR-99021 is a highly specific glycogen synthase kinase-3 (GSK-3) inhibitor which can inhibit both isoforms with IC50 of 10 nM (GSK-3α) and 6.7 nM (GSK-3β).

CHIR-99021 was proved to promote self-renewal and maintain pluripotency of both B6 and BALB/c ES cells via stabilizing the downstream effectors like c-Myc and β -catenin[1]. In J1 mESC cells, CHIR-99021 played an important role in maintaining the colony morphology as well as the self-renewal when combined with leukemia inhibitory factor (LIF). CHIR-99021 has shown to regulate multiple signaling pathways which involve Wnt/β-catenin, TGF-β, Nodal and MAPK, and the expression of epigenetic regulatory genes like Dnmt3l[2].

CHIR-99021 has been demonstrated to promote DN3 thymocytes proliferation and differentiation in the absence of pre-TCR signaling, Notch1 signaling or CXCL12[3]. However, study has also found that higher concentration (10 ?M but not 1 ?M or 3 ?M) of CHIR99021 might selectively inhibit differentiation by activating IL-7 signaling pathway[3].

References:
1. Ye S1, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P. Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains. PLoS One. 2012;7(4):e35892.
2. Wu Y1, Ai Z, Yao K, Cao L, Du J, Shi X, Guo Z, Zhang Y. CHIR99021 promotes self-renewal of mouse embryonic stem cells by modulation of protein-encoding gene and long intergenic non-coding RNA expression. Exp Cell Res. 2013
3. PLoS One. 2013;8(3):e58501. doi: 10.1371/journal.pone.0058501. Epub 2013 Mar 20. Schroeder JH1, Bell LS, Janas ML, Turner M. Pharmacological inhibition of glycogen synthase kinase 3 regulates T cell development in vitro. PLoS One. 2013;8(3):e58501.