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價(jià)格:電議
所在地:上海
型號(hào):
更新時(shí)間:2012-08-24
瀏覽次數(shù):1330
公司地址:上海市徐匯區(qū)石龍路345弄7號(hào)樓401室
唐小姐(女士)
異硫氰酸熒光素 (fluorescein isothiocyanate, FITC) FITC純品為黃色或橙黃色結(jié)晶粉末,易溶于水和酒精溶劑。有兩種異構(gòu)體,其中異構(gòu)體Ⅰ型在效率、穩(wěn)定性與蛋白質(zhì)結(jié)合力等方面都更優(yōu)良。FITC分子量為389.4,zui大吸收光波長(zhǎng)為490~495nm,zui大發(fā)射光波長(zhǎng)為520~530nm,呈現(xiàn)明亮的黃綠色熒光。FITC在冷暗干燥處可保存多年,是目前應(yīng)用廣泛的熒光素。其主要優(yōu)點(diǎn)是人眼對(duì)黃綠色較為敏感,通常切片標(biāo)本中的綠色熒光少于紅色。
Description
Application Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.
Properties
Sigma F7250 Fluorescein isothiocyanate isomer I
suitable for protein labeling, ≥90% (HPLC), powder
Synonym: FITC, Fluorescein 5-isothiocyanate
Molecular Weight: 389.38
Beilstein Registry Number: 1407295
CAS Number: 3326-32-7
Linear Formula: C21H11NO5S
EC Number: 222-042-0
MDL number: MFCD00005063
PubChem Substance ID: 24894933
assay: ≥90% (HPLC)
form powder
fluorescence λex 492 nm; λem 518 nm (green)
mp >360 °C(lit.)
suitability suitable for protein labeling
storage temp. 2-8°C
Safety
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard Codes Xn
Risk Statements 42/43
Safety Statements 22-36/37-45
WGK Germany 3