上海藍(lán)基生物科技有限公司致力于打造中國(guó)頗具實(shí)力的elisa試劑盒和抗體生產(chǎn)商，主營(yíng)生產(chǎn)elisa試劑盒和抗體；試劑品種齊全，品質(zhì)保證，良好的售后服務(wù)體系和購(gòu)買產(chǎn)品的很好的獎(jiǎng)勵(lì)制度；

       同時(shí)又是一家專業(yè)經(jīng)營(yíng)和代理國(guó)際品牌的生物、儀器、試劑耗材的公司，生物引擎 在廣大用戶的幫助和支持下，經(jīng)過不懈的努力，已成為國(guó)內(nèi)生命科學(xué)領(lǐng)域產(chǎn)品的主要供應(yīng)商之一，代理許多國(guó)際先進(jìn)水平的高科技產(chǎn)品，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)、診斷等多個(gè)研究及應(yīng)用領(lǐng)域。公司的服務(wù)和業(yè)務(wù)網(wǎng)絡(luò)遍及全國(guó)，在同行獲得一致好評(píng)。 特別是細(xì)胞培養(yǎng)產(chǎn)品更是首屈一指，美國(guó)原裝進(jìn)口原代細(xì)胞，專業(yè)培養(yǎng)基，常用傳統(tǒng)培養(yǎng)基，細(xì)胞培養(yǎng)用的相關(guān)試劑，氨基酸，抗生素，維他命類產(chǎn)品品種齊全，試劑耗材種類繁多。并代理國(guó)內(nèi)外許多著名品牌等等。 公司的規(guī)模和產(chǎn)品種類日益擴(kuò)大和更新，生物引擎 我們一直秉承著代理優(yōu)質(zhì)品牌的產(chǎn)品，服務(wù)好每一位顧客，將最新最先進(jìn)的科學(xué)技術(shù)和方法推薦顧客的服務(wù)宗旨為廣大客戶提供各類服務(wù)，產(chǎn)品、技術(shù)咨詢、國(guó)際國(guó)內(nèi)信息，每年組織大批員工出國(guó)學(xué)習(xí)，掌握國(guó)際先進(jìn)的技術(shù)和信息。公司的理念是：信譽(yù)第一、質(zhì)量第一、品牌第一，打造優(yōu)良的服務(wù)及代理品牌：藍(lán)色基因BlueGene; 這里匯集了國(guó)際國(guó)內(nèi)先進(jìn)知名品牌，為您提供全方位的產(chǎn)品及服務(wù)，產(chǎn)品價(jià)格極具競(jìng)爭(zhēng)力。 最后，感謝您對(duì)我們的支持和信賴！同時(shí)也祝您的事業(yè)更上一層樓！讓我們?cè)诮窈蟮娜兆永锕餐M(jìn)步，共創(chuàng)佳績(jī)！ 主要代理產(chǎn)品：試劑盒：ELISA試劑盒、免疫組化試劑盒、分子生物學(xué)試劑盒、細(xì)胞凋亡試劑盒、生化試劑盒。 抗體：進(jìn)口原裝抗體、進(jìn)口分裝抗體、單克隆抗體、多克隆抗體、單標(biāo)抗體、雙標(biāo)抗體、多標(biāo)抗體、一抗、二抗等等 免疫組化抗體、免疫熒光抗體、流式細(xì)胞抗體、免疫細(xì)胞化學(xué)抗體；辣根標(biāo)記、過氧化物酶標(biāo)記、PE、Cy3、Cy5標(biāo)記等等細(xì)胞：原裝進(jìn)口細(xì)胞、國(guó)產(chǎn)腫瘤細(xì)胞、國(guó)產(chǎn)正常細(xì)胞、腫瘤耐藥細(xì)胞 耗材：細(xì)胞培養(yǎng)耗材、普通實(shí)驗(yàn)耗材。 生化試劑：原裝進(jìn)口生化試劑、進(jìn)口分裝生化試劑、國(guó)產(chǎn)生化試劑。

INTENDED USE

This B.G HIS ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHIS in the sample, thisHIS ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHIS concentration. The concentration ofHIS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PRINCIPLE OF THE ASSAY

ThisHIS enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHIS. Standards or samples are then added to the microtiter plate wells andHIS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHIS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHIS are added to each well to “sandwich” theHIS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHIS and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

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